扬州大学学报（农业与生命科学版）2013，Vol.34Issue(1)：68-72,5.
大豆子叶节再生体系的建立
Establishment of regeneration system of soybean cotyledonary node
摘要
Abstract
In order to establish a cotyledonary node regeneration system for soybean, the cotyledonary nodes of 5 soybean genotypes were chosen as explants. Xiaolidou (Dongnong 50), as one of the genotypes, was screened out with the maximum induction rate of multiple shoots, which was then adopted for the selection of sterilizing methods, 6-BA concentrations on regeneration of cotyledonary node including germination of seedlings and multiple shoots induction, IBA concentrations on root regeneration. The results indicated that chorine gas seed sterilization would be the best, and seedlings germinated in the medium with 1. 0 mg · L-1 6-BA were suitable as explants. The optimal concentration of 6-BA in multiple shoots induction medium was 1. 67 mg · L-1. The optimal concentration of IBA in root regeneration medium was 1.0 mg · L-1.关键词
大豆/子叶节/丛生芽Key words
Glycine max (L. ) Merr/ cotyledonary node/ multiple shoots分类
农业科技引用本文复制引用
刘银,史秀岚,王静磊,刘琪迩,王幼平..大豆子叶节再生体系的建立[J].扬州大学学报（农业与生命科学版）,2013,34(1):68-72,5.基金项目
国家转基因生物新品种培育科技重大专项(2009ZX08004-002B) （2009ZX08004-002B）
