扬州大学学报（农业与生命科学版）2013，Vol.34Issue(1)：89-94,6.
HarpinEa和BTH诱导厚皮甜瓜抗菌物质的研究
Study on antifungal substances induced by HarpinEa and BTH in muskmelons
摘要
Abstract
Muskmelon (cv. Yindi) seedlings were sprayed with 50 mg o L-1 Harpin and 100 mg · L-1 BTH, respectively , to study the effects on the synthesis of antifungal substances during growth period. Antifungal compounds in leaves were used with methanol-dichlormethane and separated by thin layer chromatography (TLC). Alternaria alternata was regarded as indicator fungus. Antifungal compounds in inhibition zones were identified by Gas Chromatography and Mass Spectrum (GC-MS). The result indicated that some kinds of preformed antifungal compounds existed in leaves of muskmelon, such as bis (2-methylpr) 1, 2-benzenecarboxylic acid, bis (2-ethylhexyl) dioctyl ester, 1, 2-benzenedicarboxylic acid, dibutyl ester, 1,2-benzenecarboxylic acid, methyl ester and 9,12,15-octadecatrienoic acid. The species and contents of antifungal compounds significantly increased by elicitor treatments that could induce not only innate antifungal compounds but also novel antifungal compounds. Both Harpin and BTH could restrain Erysiphe cichoracearum expansion in leaves and could significantly decrease disease index of powdery mildew and downy mildew in muskmelon.关键词
厚皮甜瓜/Harpin/BTH/抗菌物质/TLC生物检测Key words
muskmelon/ Harpin/ BTH/ antifungal compounds/ TLC bioassay分类
农业科技引用本文复制引用
张咏梅,毕阳,魏晋梅,程李香..HarpinEa和BTH诱导厚皮甜瓜抗菌物质的研究[J].扬州大学学报（农业与生命科学版）,2013,34(1):89-94,6.基金项目
教育部博士点科研基金资助项目(2009620211000) （2009620211000）
