南京师大学报（自然科学版）2013，Vol.36Issue(1)：68-73,6.
籼稻不同品种成熟胚的组培发育特性及其超微结构的观察
Comparison of Ultrastructure of Callus Induction in Indica Rice With Different Callus Induction Rates
摘要
Abstract
Mature embryos from 3 Indica rice varieties as explants were implanted in the induction medium( M8+30 g/L Sucrose+10g/LAgar+5 g/L Sorbitol+2 mg/L2,4-D+2 mg/L6-BA+l mg/L ABA+0. 5 mg/L KT+1 mg/LNAA)and the callus inductivities of these materials were different significantly at 0. 01 level,respectively. At the same time, the callus incubated for 9 d and 15 d were observed by a transmission electron microscope, and the callus' browning rate was recorded at the same time. The results showed that the 3 rice materials developed differently at the same induction period. The callus cells from Yangfuxian 6 developed fast,while those from Hui 76 developed relatively slow,and the development of callus cells from Huanghuazhan was the slowest. The performances in callus ultrastructure were also consistent with their cell development periods. It suggested that the callus induction in Indica rice had close relation with their cell development rates, physiological functions and senescences as well. The callus browning was also related to the accumulation period of some black substance and the development of their vacuoles. The rate of callus induction in Indica rice would be increased by adding cytokinin in medium for callus induction.关键词
籼稻/成熟胚/愈伤组织/超微结构Key words
Indica rice,mature embryo,callus tissue,ultrastructure分类
农业科技引用本文复制引用
田亲亲,阎丽娜,陆长梅,李霞,魏晓东,方先文..籼稻不同品种成熟胚的组培发育特性及其超微结构的观察[J].南京师大学报（自然科学版）,2013,36(1):68-73,6.基金项目
江苏省自主创新课题[CX(11)1020]、国家自然科学基金项目(30871459). （11）
