南京师大学报（自然科学版）2013，Vol.36Issue(1)：85-89,5.
姬菇多糖对急性酒精性肝损伤小鼠的肝组织超微结构的影响
Effects of Polysaccharides from Pleurotus Cornucopiae on Ultrastructure Change of Acute Alcoholic Injuried Liver in Mice
摘要
Abstract
Objective: The present study investigated the protective effect of polysaccharide from Pleurotus cornucopiae (PCNP)on electron microscopic changes in acute alcoholic hepatic injury in mice. Methods;The 84 mice were randomly divided into blank control group, alcoholic model groups. The mice in alcoholic model groups were administered by gavage with BIFENDATE(150 mg/kg·bw)or PCNP( (100,200,400)mg/kg·bw)for 30 d before the administration with dose of 50% alcohol(12 mL/kg·bw). After the last alcohol treament for 12 h,in order to observe the ultrastructural changes of liver cells, all mice were dislocation death to collect samples for preparing ultrathin section for electron microscopy. Result: In model group, hepatic cells exhibited in different degrees of karyopycnosis and the nuclear membranes were irregular;The pathological changes in mitochondria had been clearly observed. In the hepatic cell, vacuoles can be seen and glycogen was increased. The rough endoplasmic reticulum were found as expansion and degranulation; Many large lipid droplets were found around the nucleus and microvilli of bile duct lost. In the PCNP groups, karyolemma were uneven. There were less expansion of mitochondria and less glycogen deposition compared with other groups;In addition, endoplasmic reticulum was not obvious outspread and lipid droplets reduced in cells. Conclusion: It can be seen that PCNP has the protective effect on hepatic ultrastructure induced by alcohol.关键词
姬菇多糖/急性肝损伤/肝组织/超微结构Key words
Pleurotus cornucopiae polysaccharides,acute liver injury,liver tissue,ultrastructure分类
轻工纺织引用本文复制引用
陶明煊,周斌,郭永月,赵云霞..姬菇多糖对急性酒精性肝损伤小鼠的肝组织超微结构的影响[J].南京师大学报（自然科学版）,2013,36(1):85-89,5.基金项目
江苏省高校自然科学基础研究项目(10KJD 550003)、江苏省普通高校研究生科研创新计划项目(CXLX11_0895). （10KJD 550003）
