南京师大学报（自然科学版）2013，Vol.36Issue(1)：94-98,5.
GGPPS多克隆抗体的制备与鉴定
Preparation and Identification of Polyclonal Antibodies of GGPPS
摘要
Abstract
To construct the prokaryotic expression vector of human GGPPS gene and prepare the specific polyclonal antibody against GGPPS. Construct the prokaryotic expression vector of pGEX - 4T - GGPPS by DNA recombination technology and induce the expression of GGPPS fusion protein. The fusion protein was purified and injected into rabbits to produce polyclonal antibody. The prokaryotic expression vector of pGEX-4T-GGPP5 was constructed and the antibody of GGPPS was obtained. The liter of the antiserum was 1:32 000. The specific polyclonal antibody against GGPPS was successfully prepared. The polyclonal antibodies preparation against GGPPS provided a good tool for us to study the function involvement of GGPPS.关键词
GGPPS/原核表达/融合蛋白/多克隆抗体Key words
GGPPS,prokaryotic expression,fusion protein,polyclonal antibody分类
生物科学引用本文复制引用
王江南,丁尧,来珊珊,陈卫波,余德才,李朝军,薛斌..GGPPS多克隆抗体的制备与鉴定[J].南京师大学报（自然科学版）,2013,36(1):94-98,5.基金项目
国家自然科学基金(31171306)、江苏省自然科学基金(BK2011568)、国家自然科学基金(30700394). （31171306）
