热带作物学报2013，Vol.34Issue(3)：429-432,4.DOI:10.3969/j.issn.1000-2561.2013.03.008
甘蔗单糖转运蛋白基因ShPST2a克隆及亚细胞定位
Cloning and Subcellular Localization Analysis of Monosaccharide Transporter in Sugarcane
郭婷 1赵婷婷 1王俊刚 1冯翠莲 1杨本鹏 1杨乃博 1张树珍1
作者信息
摘要
Abstract
ShPST2a gene was amplified by RT-PCR from mature stalks of sugarcane based on ShPST2a gene sequence of sugarcane variety Q117 in Genbank. Subcellular localization was then used to study the function of ShPST2a. The plant expression vector pCAMBIA-ShPSt2a with green fluorescent proteins (GFP) was constructed and transformed into onion epidermal cells via gene gun method. GFP fluorescence observation indicated that the ShPST2a-GYP fusion protein was located in the plasma membrane, thus ShPST2a encoding protein is a kind of membrane protein.关键词
甘蔗/ShPST2a/转运蛋白/亚细胞定位Key words
Sugarcane/ ShPST2a/ Transporter, Subcellular localization分类
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郭婷,赵婷婷,王俊刚,冯翠莲,杨本鹏,杨乃博,张树珍..甘蔗单糖转运蛋白基因ShPST2a克隆及亚细胞定位[J].热带作物学报,2013,34(3):429-432,4.
