热带作物学报2013，Vol.34Issue(3)：480-485,6.DOI:10.3969/j.issn.1000-2561.2013.03.016
甘蔗茎全长cDNA文库构建及EST序列分析
Construction of Full-length cDNA Library from Sugarcane Stem and the Corresponding EST Analysis
摘要
Abstract
Sugarcane is not only the most important sugar plant, but also one of the most important energy plants in the world Based on the sugarcane stem full-length cDNA library successfully constructed by modified Oligo-capping, we obtained 283 ESTs (expressed sequence tags) of 5'end from this cDNA library by high-throughput sequencing, the average length of these ESTs was 459 bp, and 204 TUTs (tentative unique transcript, TUT) were obtained after clustering and splicing. The rate of the redundant sequence of the library was 27.9%. NCBI homologies analysis showed that these 103 TUTs (including 132 ESTs) were determined to the tags of gene with putative function, and were classified intol2 categories according to the functional classification of Arabidopsis genes, among which the largest category was genes related to protein synthesis, the others were cell structure, protein destination and storage, transcription etc.关键词
甘蔗茎/全长cDNA文库/表达序列标签(EST)Key words
Sugarcane stem/ Full-length cDNA library/ Expressed sequence taqs (EST)分类
农业科技引用本文复制引用
刘金仙,阙友雄,郭晋隆,许莉萍,陈如凯..甘蔗茎全长cDNA文库构建及EST序列分析[J].热带作物学报,2013,34(3):480-485,6.基金项目
国家现代农业(甘蔗)产业技术体系建设项目(No.CARS-20) （甘蔗）
国家自然科学基金项目(No.31101196) （No.31101196）
福建省自然科学基金项目(No.2011J05094) （No.2011J05094）
福建省教育厅科技项目(No.JA11267). （No.JA11267）
