山西医科大学学报2013，Vol.44Issue(3)：185-188,4.DOI:10.3969/J.ISSN.1007-6611.2013.03.005
曲古霉素A对SGC7901/ADR细胞系耐药逆转机制的研究
Effect of TSA on the drug-resistance reversion in SGC7901/ADR
王丹 1孙红坤 1唐青 1杨晓丹 1乔文1
作者信息
摘要
Abstract
Objective To investigate the reversal effect of trichostain A (TSA) , a histone deacetylase inhibitor, in the gastric cancer in vitro,and to explore its mechanism. Methods ①MTT assay was used to calculate the drug-resistance index of the SGC7901/ADR and the reversion drug-resistance index after giving TSA. ②The cells were divided into blank group, 1. 5 nmol/L TSA group, and 12 nmol/L TSA group. The cells were treated respectively for 48 h and 72 h. Then the flow cytometer was used to detect the adriacin (ADR) density in the SGC7901/ADR. RT-PCR was employed to detect the MDR1 gene expression in the SGC7901. Results TSA exerted obvious reversion effects on the SGC7901/ADR. The drug-resistance index obtained by MTT method was 29.51 ,and the reversion drug-resistance index after giving TSA was 1.95. Flow cytometry results showed that the adriacin density in the SGC7901/ADR obviously increased after giving TSA in a time- and dose-dependent manner. RT-PCR results showed that the MDR1 gene expression in the SGC7901/ADR obviously decreased after giving TSA. Conclusion TSA has a distinct reversion drug-resistance effect on the SGC7901/ADR,which may be implemented by downregulating the MDR1 gene expression and increasing the ADR density in the SGC7901/ADR.关键词
TSA/胃癌/多药耐药/MDR1基因Key words
trichostain/ gastric cancer/ multidrug resistance/ MDR1 gene分类
医药卫生引用本文复制引用
王丹,孙红坤,唐青,杨晓丹,乔文..曲古霉素A对SGC7901/ADR细胞系耐药逆转机制的研究[J].山西医科大学学报,2013,44(3):185-188,4.
