天然产物研究与开发2013，Vol.25Issue(3)：358-362,5.
近红外光谱法快速测定白芍中芍药苷含量
Rapid Determination of Paeoniflorin in Paeoniae Radix Alba by Near-Infrared Spectroscopy
摘要
Abstract
The objective of the present study was to establish an analytical method for the rapid determination of paeoniflorin in Paeoniae Radix Alba by near-infrared spectroscopy. The near-infrared spectral data of 106 samples was collected by Nicolet 6700 NIR spectroradiometer and the data of the concentrations of paeoniflorin were obtained by HPLC. A mul-tivariate calibration model was then developed by partical least square ( PLS) analysis using HPLC analytical data as reference. The developed model was used to predict the concentration of paeoniflorin in unknown samples. The results showed that the correlation coefficients (R2) of the developed calibration model was 0.99395; the root-mean-square error of cross-validation (RMSECV) was 0. 33068; the root-mean-square error of calibration (RMSEC) was 0. 0563; the root-mean-square error of prediction (RMSEP) and the average rate of recovery were 0.0756 and 100.07%. The method was simple, non-polluted and accurate. It can be applied for the fast determination of large quantities of samples.关键词
近红外光谱法/偏最小二乘法/白芍/芍药苷/快速测定Key words
near-infrared spectroscopy/partial least square/Paeoniae Radix Alba/paeoniflorin/rapid determination分类
医药卫生引用本文复制引用
白雁,李小庆,雷敬卫..近红外光谱法快速测定白芍中芍药苷含量[J].天然产物研究与开发,2013,25(3):358-362,5.基金项目
河南省教育厅科技攻关项目(2008A360016) （2008A360016）
河南省重大公益科研项目(081100912500) （081100912500）
