新疆农业科学2013，Vol.50Issue(3)：490-498,9.DOI:10.6048/j.issn.1001-4330.2013.03.016
响应面法优化亚麻籽粕不溶性膳食纤维提取工艺
Response Surface Methodology for Optimization of Extraction Process of Insoluble Dietary Fiber from Flaxseed Meal
摘要
Abstract
[Objective] The purpose of this study is to evaluate the optimum extraction conditions of insoluble dietary fiber in linseed meal by response surface analysis method. [Method] Linseed meal was hydrolyzed with alkaline protease. On the basis of single factor test, the hydrolysis temperature, time, enzyme amount (mass fraction) and the ratio of solid -to -liquid were selected as response variables, the insoluble dietary fiber extraction rate was regarded as response value, and insoluble dietary fiber extraction rate and response variables regression equation were established by Box - Behnken experimental design and response surface analysis method, and the optimum extraction conditions were determined. [Result] In the quadratic model of the extraction rate, the temperature, time and enzyme amount had significant effect in a linear equation and the temperature, ratio and enzyme amount had significant effect in quadratic term. [ Conclusion] The optimum extraction conditions were hydrolysis temperature 55℃ , enzymatic hydrolysis time was 4h, the ratio of solid to liquid was 1:20 and the enzyme amount was 9%. Under this condition, the water - insoluble dietary fiber yield was 52.05% , which was consistent with the predictive value of 52.51%.关键词
亚麻籽粕/不溶性膳食纤维/响应面分析法/优化Key words
flaxseed meal/insoluble dietary fiber/response surface methodology/optimization分类
农业科技引用本文复制引用
李可,侯伟伟,秦娜娜,李天兰,张双双,杨海燕..响应面法优化亚麻籽粕不溶性膳食纤维提取工艺[J].新疆农业科学,2013,50(3):490-498,9.基金项目
校企合作项目 （）
