新疆农业科学2013，Vol.50Issue(3)：524-529,6.DOI:10.6048/j.issn.1001-4330.2013.03.020
硅胶干燥罗布麻叶片DNA提取及RAPD反应体系建立
Extraction of Apocynum Genomic DNA and Establishment of RAPD System
摘要
Abstract
[Objective] The purpose of this project is to obtain the optimal RAPD reaction system that might apply to apocynum. [Method] Apocynum in Xinyuan County is used as experimental materials and the genomic DNA was extracted from young leaves of Apocynum by means of Tiangen Kit until sufficient RAPD (Random amplified polymorphic DNA) is got to carry out analysis of Apocynum. [Result] Through single factor experiments, The best RAPD reaction system optimized was; Mg2+ (2.0 mmol/L) , dNTPs(0. 5 mmol/ L), primer(0.4 mmol/L) 、DNA(0. 3 U), Taq DNA polymerase 1 L, 10 × buffer 1 L,10 L PCR solution;The RAPD PCR was programmed by a cycle for 5 min at 941, followed by 42 cycles for 30s at 94℃, 30s at 38℃: and 2 min at 72℃, and finally by a cycle for 2 min at 72℃. [ Conclusion ] The obtained DNA is of high quality, which applies to RAPD analysis.关键词
罗布麻/RAPD体系/DNA提取Key words
Apocynum/ RAPD reaction system/ draw of DNA分类
农业科技引用本文复制引用
苏前,吕杰,任曼丽,冉启洋,张雪妮,吕光辉..硅胶干燥罗布麻叶片DNA提取及RAPD反应体系建立[J].新疆农业科学,2013,50(3):524-529,6.基金项目
国家自然科学重点基金(41130531) （41130531）
