云南农业大学学报2013，Vol.28Issue(2)：242-246,5.DOI:10.3969/j.issn.1004-390X(n).2013.02.017
野生紫斑百合高效离体再生体系的建立
Establishment of an Efficient Micro-propagation System for Wild Lilium nepalense D.Don
摘要
Abstract
The experiment was conducted to establish a high efficient plant regeneration system for wild Lilium nepalense D. Don. The scales of L. nepalense as explants were cultured in basal MS medium, and the effect of different hormones and different concentration ratios on scales differentiation, multiplication of adventitious buds and adventitious roots formation. Results showed that callus was induced from scales, and the optimal medium for callus induction was MS + 0. 5 mg/L TDZ + 0. 5 mg/L NAA and the callus induction rate was 45. 00% ; the optimal medium for multiplication of adventitious buds was MS + 1 mg/L 6-BA + 0. 5 mg/LNAA and the multiplication rate of adventitious buds was 72. 58% ; the best medium for root induction was 1/2 MS +1.5 mg/L IBA and the root induction rate was 93. 33%.关键词
野生紫斑百合/组织培养/植株再生Key words
wild Lilium nepalense D. Don/ tissue culture/ plant regeneration分类
农业科技引用本文复制引用
吴英英,许昌慧,唐军荣,赵雁鸣,张雪娟,辛培尧,何承忠..野生紫斑百合高效离体再生体系的建立[J].云南农业大学学报,2013,28(2):242-246,5.基金项目
西南林业大学面上基金项目(111146) （111146）
云南省教育厅基金项目(2012J049) （2012J049）
西南林业大学林木遗传育种校级重点学科(XKX200904). （XKX200904）
