烟台大学学报（自然科学与工程版）2013，Vol.26Issue(2)：106-110,5.
海生红藻多管藻R-藻蓝蛋白亚基组成及特性
Subunit Components and Characteristics of R-phycocyanin in Marine Red alga Polysiphonia urceolata
摘要
Abstract
R-phycocyanin is isolated and purified from the phycobiliprotein extract of marine red alga Polysiphonia urceolata using gel filtration through Sephadex G-150 and ion exchange chromatography on DEAE-Sepharose FF. The purified R-PC appears as a single band in native PAGE and in native isolectric focusing (IEF) gels with pI = 5. 6. Subunit analysis of the R-PC using SDS-PAGE, denaturing IEF and two-dimensional electrophoresis demonstrates that the R-PC has one α subunit α18.2 6.5 with molecular mass of 18. 2 kD and pI of 6. 5, and contains four β subunits with molecular mass of 20. 0 kD and 20. 9 kD and pI of 5. 5 and 5. 6, β20.0 5.5, β20.0 5.6 and β20.0 5.5. These results provide valuable experimental information for improving the investigation of R-PC structure and function.关键词
多管藻/R-藻蓝蛋白/亚基分析/二维电泳Key words
Polysiphonia urceolata/ R-phycocyanin/ subunit analysis/ two-dimensional electrophoresis分类
生物科学引用本文复制引用
曲艳艳,王玉,魏星,赵明日,龚雪琴,孙力..海生红藻多管藻R-藻蓝蛋白亚基组成及特性[J].烟台大学学报（自然科学与工程版）,2013,26(2):106-110,5.基金项目
国家自然科学基金资助项目(30571720和40976083). （30571720和40976083）
