中国病理生理杂志2013，Vol.29Issue(3)：418-424,7.DOI:10.3969/j.issn.1000-4718.2013.03.007
Bmi-1促人胶质瘤SNB19细胞生长恶化的作用机制
Effects of Bmi-1 on progression of human glioma SNB19 cells
摘要
Abstract
AIM; To study the effects of Bmi-1 on the progression of human glioma SNB19 cells. METHODS; Human glioma SNB19 cells stably overexpressing Bmi-1 were established, and Bmi-1 was knocked down using RNA interference (RNAi) methods. The proliferation, invasion and angiogenesis of SNB19 cells were detected by MTT, Tran-swell assay and tube formation. The activity of NF-κB was detected by dual-luciferase report assay and immunofluorescence cell staining. The NF-κB downstream gene expression was detected by real-time PCR. RESULTS; Bmi-1 dramatically increased the proliferation, invasiveness and angiogenesis of SNB19 cells. NF-κB transcriptional activity and NF-κB downstream gene expression were significantly increased in the glioma cells which overexpressed Bmi-1. The proliferation, invasion and angiogenesis abilities of Bmi-1-overexpressing cells were dependent on the activity of NF-κB. CONCLUSION; Bmi-1 may play an important role in the development of human glioma SNB19 cells, which might be correlated with NF-κB pathway activation and the relevant gene expression.关键词
Bmi-1蛋白/神经胶质瘤/NF-κBKey words
Bmi-1 protein/ Glioma/ NF-kappa B分类
医药卫生引用本文复制引用
蒋丽丽,杨昌山,赵灿国..Bmi-1促人胶质瘤SNB19细胞生长恶化的作用机制[J].中国病理生理杂志,2013,29(3):418-424,7.基金项目
国家自然科学基金资助项目(No.81101680) （No.81101680）
广州医学院青年基金资助项目(No.2010C11) （No.2010C11）
