中国病理生理杂志2013，Vol.29Issue(3)：515-520,6.DOI:10.3969/j.issn.1000-4718.2013.03.023
雌激素缺乏导致的骨质疏松发病过程中骨髓间充质干细胞FasL表达降低对CD4+T淋巴细胞凋亡的影响
Effect of FasL expression in bone marrow mesenchymal stem cells on CD4+ T-lymphocyte apoptosis during estrogen deficiency-induced osteoporosis
摘要
Abstract
AIM: To investigate the effect of FasL expression in bone marrow mesenchymal stem cells (BMMSCs) on CD4+ T-lymphocyte apoptosis during osteoporosis (OP) under the condition of estrogen deficiency. METHODS: An animal model of OP was established by ovariectomy (OVX, bilateral ovarian resection) in 8-week-old healthy female mice. T-lymphocytes were divided into OVX group, sham group and estradiol-stimulated group, and co-cultured with the BMMSCs from the mice. The comparison of CD4+T-lymphocyte apoptosis among the 3 groups was performed. The level of FasL expression was detected by Western blotting and real-time PCR. RESULTS: The apoptosis of CD4 + T-lymphocytes was significantly higher in sham group than that in OVX group. The expression of FasL at mRNA and protein levels was lower in OVX group than that in sham group, and was increased by estrogen in a concentration-dependent manner. CONCLUSION; Expression of FasL is regulated by estrogen in BMMSCs. The change of FasL affects apoptosis of CD4+ T-lymphocytes, which may be an important mechanism in the pathogenesis of postmenopausal osteoporosis.关键词
雌激素/骨质疏松/骨髓间充质干细胞/Fas配体Key words
Estrogen/ Osteoporosis/ Bone marrow mesenchymal stem cells/ Fas ligand分类
医药卫生引用本文复制引用
邵秉一,于洋,付欣,廖立,杨德琴,金岩..雌激素缺乏导致的骨质疏松发病过程中骨髓间充质干细胞FasL表达降低对CD4+T淋巴细胞凋亡的影响[J].中国病理生理杂志,2013,29(3):515-520,6.基金项目
国家基础研究项目(973项目)(No.2011CB964700) （973项目）
