中国临床药理学与治疗学2013，Vol.18Issue(3)：247-252,6.
新型维甲酸衍生物ATPR对肿瘤细胞株SKOV3增殖及分化的影响
Effects of a new the retinoic acid derivatives ATPR on proliferation and differentiation of SKOV3 tumor cell
摘要
Abstract
To explore the effect of the 4-amino-2-trifluoromethyl-phenyl retinate ( ATPR) on the proliferation and differentiation of SK0V3 tumor cell in vitro. METHODS: Cell proliferation was assessed by MTT assay and growth curve of cells; morphologic changes were observed after Giemsa staining using inverted phase contrast microscope; CA125 were measured by ELISA; the cell cycle were analyzed by Flow Cytometry (FCM). RESULTS: The growth of SKOV3 cells was inhibited in a does-dependent manner after the treatment with ATPR. The phanero depressant effect appeared at 48 h, but it was significant after 72 h. Cell morphous was observed to be mature in inverted microscope. The content of CA125 decreased. The proportion of cells in G0/G1 phase increased while the cells in S phase decreased. Cell cycle progression was blocked in the G0/G1 phase. CONCLUSION: ATPR could inhibit the proliferation and induce differentiation in some degree. The direction of differentiation is still need further study.关键词
ATPR/SKOV3细胞/增殖/诱导分化Key words
ATPR/ SKOV3 cell line/ Proliferation/ Induced differentiation分类
医药卫生引用本文复制引用
吴繁荣,洪凡青,陈飞虎..新型维甲酸衍生物ATPR对肿瘤细胞株SKOV3增殖及分化的影响[J].中国临床药理学与治疗学,2013,18(3):247-252,6.基金项目
国家"重大新药创制"科技重大专项(2011ZX09401-021) （2011ZX09401-021）
国家自然科学基金(81100301) （81100301）
