中国临床药理学与治疗学2013，Vol.18Issue(3)：263-270,8.
西红花酸对溃疡性结肠炎大鼠模型的作用及其机制研究
Effects of crocetin on ulcerative colitis in rats and the mechanism
陶赟 1龚国清 1钱之玉 1成文媛 1王欣慧1
作者信息
摘要
Abstract
To determine the effect of crocetin on ulcerative colitis(UC) induced by tri-nitrobenzene sulfonic acid(TNBS)in rats, and to explore the possible mechanism. METHODS: UC was induced by TNBS and ethanol in rats. After crocetin was given orally, the colon muco-sa damage idex and histopathological score were determined, and the levels of MPO, MDA and SOD were measured. Tumor necrosis factor-a (TNF-a) and Interleukin-1β (IL-1β) in colon were detected by enzyme-linked immunosorbent assay(ELISA). Toll-like receptor4(TLR4) mR-NA and nuclear factor-κBp65 (NF-κBp65) mR-NA expression were examined by Q-PCR. Im-munohistochemistry staining was used to observe the expression of TLR4 and NF-κBp65. RESULTS: Crocetin greatly attenuated the sever-
ity of colonic gross lesions and reduced the histopathological scores. MPO activity and MDA concentration in colon were significantly increased, SOD activity was reduced in UC rats. Crocetin ameliorated these changes. Crocetin reduced the levels of TNF-a and IL-1β in colon. The expression of TLR4 and NF-κBp65 in colon were attenuated by crocetin. CONCLUSION: Crocetin can effectively attenuate the inflammatory reaction in UC rats, which may be due to the antioxidation and the inhibition of TLR4/ NF-κB signal pathway.关键词
溃疡性结肠炎/西红花酸/Toll样受体4/NF-κBKey words
Ulcerative colitis/ Crocetin/ TLR4/NF-κB分类
医药卫生引用本文复制引用
陶赟,龚国清,钱之玉,成文媛,王欣慧..西红花酸对溃疡性结肠炎大鼠模型的作用及其机制研究[J].中国临床药理学与治疗学,2013,18(3):263-270,8.
