中国临床药理学杂志2013，Vol.29Issue(4)：299-301,3.
RP-HPLC法测定人血浆和唾液中奥卡西平活性代谢物的浓度
Determination concentration of active metabolite of oxcarbazepine in human plasma and saliva by RP-HPLC
冯健全 1许俊羽 2吴晔 3周颖 3崔一民3
作者信息
- 1. 北京大学第一医院,药剂科,北京100034
- 2. 北京大学药学院药事管理与临床药学系,北京100191
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the concentration of monohydroxycarbamazepine ( MHD) in human plasma and saliva. Methods The analysis was conducted using a ZORBAX Eclipse Plus C18 column and the column temperature was 40 ℃. The mobile phase consisted of methanol and water (45:55). The flow rate was 1 mL ? min-1 and the detection was performed at 254 nm. And the internal standard substance was p - Chloroacetanilide. Results Endogenous impurities does not interfere with the determination of the samples in plasma and saliva. The standard curve is linear in the range of 2 ~ 100 μg ? mL-1 (γ =0. 999 8 in the plasma, y =0. 999 8 in the saliva) , LLOQ is 2 μg ? mL-1. The RSDs of inter - day and intra - day are all less than 5% in the plasma and the extraction recovery is 72%~89%. The RSDs of inter - day and intra - day are all less than 5. 6% in the saliva and the extraction recovery is 75% ~91%. Conclusion This method is simple, sensitive, accurate and suitable for routine determination of the concentration of MHD in human plasma and saliva.关键词
奥卡西平/代谢物/高效液相色谱/血浆/唾液Key words
oxcarbazepine/metabolite/HPLC/plasma/saliva分类
医药卫生引用本文复制引用
冯健全,许俊羽,吴晔,周颖,崔一民..RP-HPLC法测定人血浆和唾液中奥卡西平活性代谢物的浓度[J].中国临床药理学杂志,2013,29(4):299-301,3.
