中国输血杂志2013，Vol.26Issue(3)：138-142,5.
番禺地区RHD变异体基因分型研究
Genotyping study of RHD variants among RhD-negative blood donors, Panyu district
摘要
Abstract
Objective To study and analyze genotyping characteristics of RHD gene variants among Panyu RhD-negative blood donors. Methods Use micro-plate method to screen RhD-negative samples among 26 172 blood donors from November 2010 to August 2011 in Panyu Blood Centre. Using anti-globulin method to identify RhD-negative screening samples , using adsorption and elution test to screen De phenotype among the above RhD-negative-identified samples, using RH gene variants genotyping assay kit (PCR-SSP) to amplify template DNA of blood donors and the amplified products were analyzed by electrophoresis. And RHD genotyping was determined according to the polyacrylamide gel electrophoresis. The samples that the serological and genotyping results were not matching were sequenced. Results 60 RhD negative samples were screened out among 26 172 blood donors,and 59 samples were confirmed RhD negative by anti-globulin method, with the negative frequency of 0. 23%. Adsorption and elution test detected out 10 cases of Del phenotype. Genotyping detected the 60 RhD-negative samples,40 cases of RHD EXONl ~ 10 deletion genotype were found, with the approximately frequency of 67. 8% , and 10 cases of RHD 1227A DEL, with the approximately frequency of 16. 9% , and 7 cases of partial D, with the approximately frequency of 11.9% , and 1 cases of RHD weak D\5 genotype, with the approximately frequency of 1. 2% , and 2 cases needed further allele sequencing. Conclusion In Panyu district, among the serological RhD-negative blood donors, RHD \221A DEL, RHD-(2-9)CE-D, RHD-CE(5)-D, RHD-CE(6-9)-D and RHD weak D15 genotype etc. were existing. Adsorption and elution test combined with PCR-SSP, could further improve the accuracy of RhD blood group identification.关键词
RHD变异体/基因分型/PCR-SSPKey words
RHD variants/ genotyping/ PCR-SSP分类
医药卫生引用本文复制引用
谢敬文,邓诗桢,严康峰,蓝文莉,莫锦政,马伟文..番禺地区RHD变异体基因分型研究[J].中国输血杂志,2013,26(3):138-142,5.基金项目
广州市2010年度科技计划:多重PCR技术筛选鉴定红细胞稀有血型研究(2010Y1-C881) （2010Y1-C881）
