中国药理学通报2013，Vol.29Issue(4)：468-473,6.DOI:10.3969/j.issn.1001-1978.2013.04.007
蛇毒神经生长因子对大鼠肝星状细胞增殖、凋亡及肝纤维化相关蛋白质表达的影响
Effects of cobra venom NGF on cell proliferation, apoptosis and liver fibrosis protein expression in rat hepatic stellate cells
摘要
Abstract
Aim To investigate the effects of cobra venom nerve growth factor ( NGF )on cell proliferation , apoptosis and the differences of protein expression in rat hepatic stellate cell ( HSC-T6 ), and to further provide the basis for the treatment of anti-liver fibrosis.Methods The experimental cells were divided into the control group( simple HSC-T6 culture ) and the experimental group ( NGF intervention ); MTT was used to test the effect of cobra venom NGF on HSC-T6 cells viability; flow cytometry was adopted to detect HSC-T6 cell apoptosis after adding the cobra venom NGF; the protein expression changes of HSC-T6 cell were observed with two-dimensional electrophoresis; the differentially expressed proteins were identified by mass spectrometry.Results MTT assay showed that NGF significantly inhibited the proliferation of HSC-T6 cells ( 5 mg·L-1 NGF inhibition ratio is 58.2% ± 1.9% ,P <0.01 ).It was also found in flow cytometry that apop- totic ratio of HSC-T6 was 21.15% ± 3.31% ( 5 mg· L-1 NGF ), which was obviously higher than in control ( 2.7% ± 1.55% , P < 0.05 ).After a comparative a-nalysis of control group and NGF group,47 differential protein spots were found, among which 22 protein spots were up-regulated and 25 protein spots were down-regulated.9 proteins were identified by mass spectrome-try, four of which were up-regulated and 5 were down- regulated.Conclusion Cobra venom NGF can inhibit cells proliferation, induce cell apoptosis, and affect the protein expression in rat hepatic stellate cell( HSC- T6).关键词
眼镜蛇毒NGF/肝星状细胞HSC-T6/肝纤维化/增殖/凋亡/流式细胞技术/双向电泳技术/质谱技术Key words
cobra venom NGF/ HSC-T6/ liver fibrosis/ proliferation/ apoptosis/ flow cytometry/ 2-DE/mass spectrometry分类
医药卫生引用本文复制引用
胡仁统,张学荣,徐瑾,罗小玲,林兴,廖明..蛇毒神经生长因子对大鼠肝星状细胞增殖、凋亡及肝纤维化相关蛋白质表达的影响[J].中国药理学通报,2013,29(4):468-473,6.基金项目
国家自然科学基金资助项目(No 81160063) （No 81160063）
广西自然科学基金资助项目(No 2011GXNSFA018268,KFJJ2010-43) （No 2011GXNSFA018268,KFJJ2010-43）
广西教育厅科研项目(No 201012MS058) （No 201012MS058）
广西中医药管理局项目(No GZKZ10-013) （No GZKZ10-013）
