中国药理学通报2013，Vol.29Issue(4)：495-500,6.DOI:10.3969/j.issn.1001-1978.2013.04.013
无创性肢体缺血预适应对不同病程糖尿病大鼠缺血/再灌注心肌的保护作用
Cardioprotection of noninvasive limb ischemic preconditioning against ischemia-reperfusion injury in myocardium with different duration of diabetes mellitus in rats
摘要
Abstract
Aim To investigate the effects of noninvasive limb ischemic preconditioning ( NLIP ) on myocardium after ischemia-reperfusion injury in streptozotocin ( STZ ) induced diabetic rats with different duration ( one-week and four-week ).Methods Rats received a single injection of STZ through the tail vein and acute diabetes mellitus of one-week and four-week duration was induced.The diabetic rats were divided randomly into sham, ischemia-reperfusion ( I/R ) , cardiac ischemic preconditioning ( CIP ) and NLIP groups.Diabetic rats in NLIP group were subjected to NLIP for 3 days.On the next day, diabetic rats in I/R, CIP, NLIP groups were subjected to myocardial ischemia-reperfusion injury.Diabetic rats in CIP group were preconditioned before ischemia.Diabetic rats in sham group were threaded a silk suture under the left coronary artery anterior descending ( LAD ) and laid up.Blood pressure and ECG were monitored.Myocardial infarct size was examined.Activities of CK-MB and LDH were detected.Results All the diabetic rats exhibited increased level of blood glucose and reduction of body weight.NLIP significantly decreased the incidence of ventricular premature contraction ( VPC ) and ventricular tachycardia ( VT ) during ischemia in diabetic rats with one-week and four-week duration, delayed onset and shortened duration of VPC in diabetic rats with one-week duration, which reached to the extent of that by CIP, and shortened duration of VPC in diabetic rats with four-week duration.Compared with diabetic rats with one-week duration, myocardial infarct size of I/R group was significantly increased in diabetic rats with four-week duration; NLIP and CIP obviously reduced myocardial infarct size in diabetic rats with one-week and four-week duration.Compared with I/R group, activities of CK-MB and LDH were decreased in NLIP and CIP groups of diabetic rats with one-week and four-week duration.Conclusion NLIP has a similar delayed cardioprotection in diabetic rats with one-week and four-week duration.关键词
无创/肢体缺血预适应/糖尿病/不同病程/缺血／再灌注/延迟相心脏保护Key words
noninvasive/ limb ischemic preconditioning/ diabetes mellitus/ different duration/ ischemiareperfusion / delayed cardioprotection分类
医药卫生引用本文复制引用
王蕾,张曦,朱学慧,张颖,王亚霓,康毅,高卫真,娄建石..无创性肢体缺血预适应对不同病程糖尿病大鼠缺血/再灌注心肌的保护作用[J].中国药理学通报,2013,29(4):495-500,6.基金项目
国家自然科学基金资助项目(No 81072631) （No 81072631）
天津市自然科学基金重点项目(No 09JCZDJC21100) （No 09JCZDJC21100）
