中国药理学通报2013，Vol.29Issue(4)：531-534,4.DOI:10.3969/j.issn.1001-1978.2013.04.019
丹酚酸B对肺上皮细胞凋亡和急性肺损伤的影响
Effects of salvianolic acid B on lung epithelial cell apoptosis and acute lung injury
摘要
Abstract
Aim To explore the effects of salvianolic acid B on apoptosis of lung epithelial cell and acute lung injury.Methods Apoptotic rates of lung epithelial cells were measured by Hoechst staining and flow cytometry.Lipopolysaccharides ( LPS ) was used to establish acute lung injury model of mice.Results Pre-treatment with salvianolic acid B decreased the apoptosis of NCI-H292 cells induced by H2O2 in a concentration-dependent manner, which was measured by Hoechst staining.Compared with the model group, salvianolic acid B concentration-dependently suppressed the apoptosis of NCI-H292 cells measured by flow cytometry.In vivo, pretreatment with salvianolic acid B by intravenous injection dose-dependently inhibited accumulation of neutrophils and macrophages in lung induced by LPS in mice.Conclusions Salvianolic acid B has significant anti-apoptotic and anti-inflammatory effects both in vitro and in vivo.These results help to further study the mechanism of action for lung inflammation.关键词
丹酚酸B/肺上皮细胞/细胞凋亡/流式细胞法/脂多糖/急性肺损伤/小鼠Key words
salvianolic acid B/ lung epithelial cells/apoptosis/ flow cytometry/ lipopolysaccharides/ acute lung injury/ mice分类
医药卫生引用本文复制引用
梁家红,张水娟,姚立..丹酚酸B对肺上皮细胞凋亡和急性肺损伤的影响[J].中国药理学通报,2013,29(4):531-534,4.基金项目
国家自然科学基金资助项目(No30973542) （No30973542）
