中国药理学通报2013，Vol.29Issue(4)：544-548,5.DOI:10.3969/j.issn.1001-1978.2013.04.022
腺苷对肿瘤坏死因子-α诱导乳鼠心肌细胞肥大及核因子-κB的影响
Effects of adenosine on tumor necrosis factor-α induced myocardial hypertrophy and the expression of nuclear factor-κB in neonatal rats
摘要
Abstract
Aim To investigate the effect of adenosine ( Ado )on cardiomyocyte hypertrophy induced by tumor necrosis factor-α ( TNF-α )and the expression of nuclear factor-ΚB( NF-ΚB ).Methods The primary cultured neonatal rat ventricular myocytes were as models , the hypertrophic cardiac myocytes were induced by TNF-α of 100 μg·L-1 , and the effect of different concentrations of Ado on cardiac hypertrophy was observed.Total protein content was assayed by the method of Bradford ; the expression of atrial natriuretic pep-tide ( ANP ) mRNA was determined by RT-PCR; the expression of p65 and that of inhibitor-κBα ( IκBα ) were measured by Western blot; the expression of ( In-terleukin-1β) IL-1β was determined by ELISA.Results Ado was able to inhibit the TNF-α-induced cardiac hypertrophy, which was expressed as the decrease of the protein content and the expression of ANP mRNA.It could also inhibit the TNF-α-induced NF-ΚB expression, which was expressed as the decrease of the p65 protein and IL-1β expression, and the increase of IΚBΑ protein.Conclusions Ado has a protective effect on TNF-α-induced cardiac hypertrophy.The mechanism may be related to the inhibition of NF-ΚB.关键词
腺苷/肿瘤坏死因子-α/心肌肥大/保护作用/核因子-κB/炎症反应Key words
adenosine/ tumor necrosis factor-α/ myocardial hypertrophy/ protective effect/ nuclear factor-ΚB / inflammatory effect分类
医药卫生引用本文复制引用
张岩,杨育红,梁灵君,孙雪芳,李洪秀,王洪新..腺苷对肿瘤坏死因子-α诱导乳鼠心肌细胞肥大及核因子-κB的影响[J].中国药理学通报,2013,29(4):544-548,5.基金项目
辽宁省科技计划项目(No 2009225010-40) （No 2009225010-40）
辽宁省教育厅创新团队项目(No 2009T064) （No 2009T064）
