中国药理学通报2013，Vol.29Issue(4)：557-562,6.DOI:10.3969/j.issn.1001-1978.2013.04.025
氯化钴诱导化学缺氧对N9小胶质细胞的损伤作用及机制
Injury effect of CoCl2-induced chemical hypoxia on N9 microglia
摘要
Abstract
Aim To investigate the injury effect of CoCl2 on N9 microglia and explore its mechanism.Methods N9 microglia was exposed to different doses of CoCl2 for 24h, the cell viability was assessed by XTT assay.Cellular morphological changes were observed under phase-contrast microscope.The MDA level in the cultured supernatant was detected with thiobarbituric acid( TBA ) method.The cell apoptosis was evaluated by Hoechst 33258 staining.The cell cycle was detected by flow cytometry ( FCM ) .Results CoCl2( 50 ~ 1 000 μmol·L-1 ) reduced the cell viability significantly ( P < 0.01 ) in a dose-dependent manner.The morphology of the cells changed obviously after CoCl2 treatment for 24h.Cell shrinkage and necrosis were observed and the cell density decreased.The morphological damage aggravated with the increase of the dose of CoCl2.TBA assay showed that the MDA content in the medium markedly increased( P < 0.05 ) after CoCl2 treatment.Hoechst 33258 staining showed that CoCl2 increased the percentage of apoptotic cells dose-dependently( P <0.01 ).FCM showed that CoCl2 blocked N9 microglia at G1/G0 phase.Conclusion CoCl2 can damage N9 microglia dose-dependently by inducing cell apoptosis, causing oxidative stress and cell cycle arrest.关键词
氯化钴/诱导/化学缺氧/N9小胶质细胞/细胞损伤/模型Key words
cobalt chloride( CoCl2 )/ induce/ chemical hypoxia/ N9 microglia/ cell damage/ model分类
医药卫生引用本文复制引用
王国红,毕凌云,李超堃,侯软玲,尹雅玲,李东亮..氯化钴诱导化学缺氧对N9小胶质细胞的损伤作用及机制[J].中国药理学通报,2013,29(4):557-562,6.基金项目
河南省2009年科技发展计划项目(No 092102310098) （No 092102310098）
复旦大学神经生物学国家重点实验室开放课题资助(No 10-10) （No 10-10）
新乡医学院重点领域开放课题资助(No ZD 2011-2) （No ZD 2011-2）
