中国药理学通报2013，Vol.29Issue(4)：573-576,4.DOI:10.3969/j.issn.1001-1978.2013.04.028
人参皂苷Rbl对阿霉素心力衰竭大鼠心肌细胞核因子-κB的影响
Effects of Ginsenosides-Rbl on nuclear factor kappa B in rats with chronic heart failure induced by adriamycin
摘要
Abstract
Aim Based on the adriamycin( Adr)-induced chronic heart failure ( CHF ), the present study elucidates the effect of Ginsenosides-Rbl ( Gs-Rb1 ) on nuclear factor kappa B ( NF-ΚB ), in vivo and in vitro.Methods CHF rats were randomly divided into Adr group ( n = 15 ) and Gs-Rb1 group ( 70 mg·kg-1·d-1 , n = 17 ), and the age-matched rats as control ( n = 15 ); in addition, cardiomyocytes were randomly divided into control group, Adr group ( 1μmol·L-1) and Gs-Rbl group ( 1 μmol·L-1 Adr +200 μmol·L-1 Gs-Rb1), too.After the above intervention was performed, the left ventricular ejection fraction ( LVEF ) was analyzed by echocardiography, in vivo, the expression of NF-ΚB, IΚBΑ, p-IκBα and NF-ΚB DNA binding activity were assayed.Results ( 1 ) LVEF was significantly improved in Gs-Rb1 group than in Adr group ( P =0.003 ).( 2 ) Both NF-κB p50 and NF-ΚB p65 were significantly down-regulated in Gs-Rbl group than in Adr group ( P <0.01 ), in vivo and ex vivo.( 3 ) NF-ΚB DNA binding activity was significantly down-regulated in Gs-Rb1 group than in Adr group ( P <0.01 ), in vivo and ex vivo.( 4 ) IΚBΑ protein was significantly up-regulated by Adr, especially by Gs-Rb1, compared with control ( P <0.01 ), in vivo and in vitro; both p-IκBα protein and p-IκBα/IκBα ratio were significantly lower in Gs-Rb1 group than in Adr group, in vivo and in vitro.Conclusion The effects of Gs-Rb1 on the improvement of CHF were partly performed by means of inhibiting NF-ΚB at least.关键词
人参皂苷Rbl/阿霉素/慢性心力衰竭/核因子-κB/核因子-κB抑制蛋白/核因子-κB DNA结合活性Key words
Ginsenosides-Rb1/ adriamycin/ chronic heart failure/ nuclear factor kappa B/ IΚB / NF-ΚB DNA binding activity分类
医药卫生引用本文复制引用
孔宏亮,苗志林,郭翠艳,孟锦..人参皂苷Rbl对阿霉素心力衰竭大鼠心肌细胞核因子-κB的影响[J].中国药理学通报,2013,29(4):573-576,4.基金项目
辽宁省自然科学基金资助项目(No 201102107) （No 201102107）
