中国医学装备2013，Vol.10Issue(3)：4-6,3.
基于生物介质的丙三醇对分子荧光强度影响的研究
Effect of glycerol on fluorescence intensity of fluorescent molecules in biomedium
摘要
Abstract
Methods: The study was designed to determine the changes of the fluorescent intensity of topotecan, Sulforhodamine B, Acridine orange and Rhodamine B in phosphate buffer saline solution with different concentration of glycerol, and the fluorescence and ultraviolet-visible absorption spectra of glycerol. Results: The results showed that glycerol itself has no fluorescence but can affect fluorescent intensity of the fluorescent molecules, Furthermore, the higher the concentration of glycerol was, the stronger the fluorescent intensity of molecules was. Conclusions: Adding glycerol to biological medium can strengthen the fluorescent intensity of molecules. Selecting high percentage of glycerol solution is an approach to improve the detection sensitivity of the above fluorescent molecules.关键词
丙三醇/生物介质/荧光分子/荧光强度Key words
Glycerol/ Biological medium/ Fluorescent molecules/ Fluorescent intensity分类
医药卫生引用本文复制引用
李俊霞,袁兰,吴后男,韩鸿宾,黄健,张殊佳..基于生物介质的丙三醇对分子荧光强度影响的研究[J].中国医学装备,2013,10(3):4-6,3.基金项目
国家自然科学基金青年科学基金项目(21101008)"生物介质中生成的和人工合成的钙磷酸盐微粒的差异及对细胞分化的影响" （21101008）
科技部十二五科技支撑计划(2012BAI15B009)"经脑间质途径的脑病诊断与治疗仪". （2012BAI15B009）
