质谱学报2013，Vol.34Issue(2)：88-95,8.DOI:10.7538/zpxb.2013.34.02.0088
基于UPLC-TOF-MS分析人参麦冬配伍后皂苷类成分的变化
UPLC-TOF-MS Based Profiling Approach to Evaluate Ginsenoside Composition in Combination of Ginseng and Radix Ophiopogonis
摘要
Abstract
An ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC-TOF-MS) based on chemical profilingapproach was used to evaluate chemical constitution between co-decoction and mixed decoction of ginseng and radix ophiopogonis. Two different kinds of decoctions, namely ginseng and radix ophiopogonis co-decoction (water extract of mixed two herbs), and ginseng and radix ophiopogonis mixed decoction (mixed water extract of each individual herbs) were prepared. The chromatography was performed using Acquity BEH C18 column, the mobile phase consisted of (A) water containing 0. 1% formic acid and (B) acetonitrile, mass spectrometry was performed on anelectrospray ionization source operating in V mode. Batches of these two kinds of decoction samples were subjected to UPLC-TOF-MS analysis, the datasets of tR-m/z pairs, ion intensities and sample codes were processed with supervised partial least squared discriminant a-nalysis (OPLS-DA) to holistically compare with the difference of these two kinds of decoction samples. Results show that the content of ginsenoside Rg1, ginsenoside Re, ginsen-oside Rb1 and malonylginsenoside Rbl are decreased, while the content of ginsenoside Rb3, malonylginsenoside Rb2, malonylginsenoside Rc and ginsenoside Rf are increased in samples of co-decoction of ginseng and radix ophiopogonis. The content of protopanaxatriol type ginsenoside decreased, while the content of protopanaxadiol type ginsenoside increased, which may be basis of chemical composition in combination of ginseng and radix ophiopogonis.关键词
人参/麦冬/配伍/超高效液相色谱-飞行时间质谱(UPLC-TOF-MS)Key words
ginseng/ radix ophiopogonis/ compatibility/ ultra performance liquid chroma-tography coupled with time-of-fight mass spectrometry (UPLC-TOF-MS)分类
化学化工引用本文复制引用
周思思,马增春,梁乾德,汤响林,王宇光,谭洪玲,肖成荣,张伯礼,高月..基于UPLC-TOF-MS分析人参麦冬配伍后皂苷类成分的变化[J].质谱学报,2013,34(2):88-95,8.基金项目
国家自然科学基金(81274127) （81274127）
国家重点基础研究发展计划(2012CB518402,2011CB505304)资助 （2012CB518402,2011CB505304）
