中药材2013，Vol.36Issue(1)：61-64,4.
云南兔儿风小极性成分GC-MS分析
GC-MS Analysis of Low Polar Components from Ainsliaea yunnanensis
摘要
Abstract
Objective:To analyze the chemical constituents of low polar components from ethanol extract of Ainsliaea yunnanensis. Methods: The air-dried stems and leaves of Ainsliaea yunnanensis were extracted with ethanol using ultrasonic extration. After removal of solvent under reduced pressure,the residue was suspended in H2O,and then partitioned sequentially with petroleum ether,EtOAc and n-BuOH, respectively. Then the petroleum ether fraction was chromatographed on normal phase silica gels eluting with a gradient increasing Me2CO in petroleum ether. Then the components of low polarity were separated from the petroleum ether-Me2CO(50:1 ~20: 1) fraction and identified by GC-MS, and the relative contents of the components were determined with area percentage method. Results; 50 constituents were identified from the low polar components of Ainsliaea yunnanensis. Conclusion:The research provides a theoretical basis for the study of chemical constituents and pharmaceutical activities of Ainsliaea yunnanensis.关键词
云南兔儿风/小极性成分/GC-MSKey words
Ainsliaea yunnanensis Franch. / Low polar components/ GC-MS分类
医药卫生引用本文复制引用
王蓉,孙珍,王阿利,院珍珍,李金杰,尚小雅..云南兔儿风小极性成分GC-MS分析[J].中药材,2013,36(1):61-64,4.基金项目
北京市教委面上项目(KM201111417001) （KM201111417001）
