中药材2013，Vol.36Issue(1)：93-95,3.
复方肝舒康抗乙肝病毒活性研究
Research of Inhibition of Compound Gan Shu Kang on Hepatitis B Virus
摘要
Abstract
Objective:To study the anti-HBV effect in vitro of each extract from compound Gan Shu Kang. Methods:The toxicity was investigated with cytopathic effect (CPE) by enzyme linked immuno-adsorbed assay (ELISA) ,the inhibitory effect of each extract on HBsAg and HBeAg secreted by 2215 cell line infected by HBV DNA was evaluated. Results:The half of the toxic concentration (TC50 ) of petroleum ether,ethyl acetate and n-butanol was 125,375 and 62. 5 μg/mL,respectively. The maximum nontoxic concentration (TC0 ) was 62. 5,125 and 31.3 μg/mL, respectively. The medium effective concentration ( IC50 ) of petroleum ether and ethyl acetate on 2215 cellular HBsAg express the inhibitory effect was 48. 6 and 14. 0 μg/mL,HBeAg was 52. 4 and 19. 7 μg/mL,while the IC50 of n-butanol for HBsAg and HBeAg was more than 125 μg/mL. The therapeutic index ( TI) of petroleum ether was 2. 57 and 2.43, ethyl acetate was 26. 7 and 19. 04,n-butanol was both less than 2. Conclusion:The anti-HBV effect of ethyl acetate extract of compound Gan Shu Kang is better than that of petroleum ether,while the n-butanol extract shows no effect.关键词
复方肝舒康/2215细胞/乙肝病毒Key words
Compound Gan Shu Kang/2215 cell/HBV分类
医药卫生引用本文复制引用
李虹霖,程桃英,鲁萍,程雅丽,赵祥..复方肝舒康抗乙肝病毒活性研究[J].中药材,2013,36(1):93-95,3.基金项目
国家自然科学基金(30870249,81173509) （30870249,81173509）
湖北省2008-2009年度中医药中西医结合科研项目课题计划(2008Z-Y02) （2008Z-Y02）
