安徽农业大学学报2013，Vol.40Issue(2)：199-202,4.
dw基因mRNA在矮小型优质肉鸡S2系不同组织的表达差异分析
Expression of sex-linked dw gene and regulation for the growth in different tissues of S2 line of dwarf chicken
摘要
Abstract
S2 line of dwarf chicken, as quality broiler, was caused by the sex-linked dwarfing (SLD) gene. In order to illustrate the influence of dw gene on the growth performance, the expression patterns of dw mRNA were examined in four growth related tissues (liver, breast muscle, thigh muscle, periosteum) at 12-week-old female and male of S2 line of SLD chicken. Results of the real time-PCR showed that dw gene was expressed in liver, breast muscle, thigh muscle and periosteum, but its expression abundance in different tissues was different, dw gene mRNA for highest level expression was in the liver of male chicken and female chicken(copy number of male chicken was 11.2±1.11 and female chicken was 10.4±0.51), and was significantly higher than that in its breast, thigh muscle and periosteum(P<0.05). The mRNA level of dw gene in the periosteum of S2 line of SLD chicken(copy number of male chicken was 1.8±0.37 and female chicken was 1.6±0.4) was significantly lower than that in liver, breast and thigh muscles(P<0.05). dw gene mRNA for highest level expression in thigh muscle was significant than that in female chicken (P<0.05).There is no obvious different between the mRNA level of dw gene in breast and thigh muscles of male chicken (P>0.05). The expression level of dw gene mRNA in liver and periosteum of female chicken has no obvious sex effects, but in breast and thigh muscles has extremely significant sex effects.关键词
矮小型优质肉鸡(S2系)/dw基因/表达/RT-PCRKey words
S2 line of dwarf chicken/ dw gene/ expression/ RT-PCR分类
农业科技引用本文复制引用
李春苗,黄华云,黎寿丰,赵振华,薛龙岗..dw基因mRNA在矮小型优质肉鸡S2系不同组织的表达差异分析[J].安徽农业大学学报,2013,40(2):199-202,4.基金项目
现代农业产业技术体系建设专项(CARS-42-Z06),江苏省农业三项工程(SXGC (2012) 276)和扬州市农业科技攻关项目(YZ20102050). （CARS-42-Z06）
