安徽农业科学2013，Vol.41Issue(5)：1911-1915,5.
镉、铜胁迫下拟南芥基因组DNA甲基化的MSAP分析
Analysis of Genomic DNA Methylation in Arabidopsis thaliana under Cd and Cu stress using Methylation-sensitive Amplification Polymorphism Technique
摘要
Abstract
[ Objective ] To study the effect of Cd and Cu stress on the genomic DNA methylation levels and states of Arabidopsis thaliana. [Method] Arabidopsis thaliana was planted in different concentration nutrient solution with 0, 0.5 mg/L Cd, 5.0 mg/L Cd, 0.5 mg/L Cu,5.0 mg/L Cu for 21 days. Genomic DNA was extracted to conduct methylation-sensitive amplification polymorphism (MSAP) analysis. [Result] MSAP rate of 0,0.5 mg/LCd, 5.0 mg/L Cd, 0.5 mg/L Cu, 5.0 mg/L Cu was 30.2% , 34.7%, 41.0%, 33.9%, 39.2%, DNA methylation polymorphism rate of 0.5 mg/L Cd, 5.0 mg/L Cd, 0.5 mg/L Cu, 5.0 mg/L Cu were 2.6% , 13.2% , 2.4% , 11.2% , respectively. [Conclusion ] Arabidopsis thaliana genome DNA methylation level and methylation polymorphism under Cd and Cu stress increases with the concentration increased obviously, the effect of Cd for the change of DNA methylation level and polymorphism is greater than Cu.关键词
拟南芥/DNA甲基化/镉胁迫/铜胁迫/MSAPKey words
Arabidopsis thaliana/ DNA-methylatio/ Cadmium(Cd) stress/ Copper(Cu) stress/ MSAP分类
农业科技引用本文复制引用
赵晓辉,徐正进,刘宛,王鹤潼,李照令,马珊珊,孙晓霞..镉、铜胁迫下拟南芥基因组DNA甲基化的MSAP分析[J].安徽农业科学,2013,41(5):1911-1915,5.基金项目
国家自然科学基金面上项目(20977095) （20977095）
辽宁省自然科学基金项目(201202224) （201202224）
国家科技重大专项(2012ZX07505-001) （2012ZX07505-001）
国家基金重点项目(40930739) （40930739）
沈阳大学区域污染环境生态修复教育部重点实验室基金资助. （）
