安徽农业科学2013，Vol.41Issue(5)：1916-1917,2164,3.
菜豆荚斑驳病毒外壳蛋白基因的克隆与原核表达
Cloning and Prokaryotic Expression of Coat Protein Gene from Bean Pod Mottle Virus
摘要
Abstract
[Objective] The paper was to clone the coat protein (CP) gene from bean pod mottle virus (BPMV) and express it in prokaryote. [ Method] The BPMV CP gene was obtained by using RT-PCR method,and it was connected to the pMD19-T Simple vector to sequence the positive clones and detect the homology between the CP gene and the known virus coat protein gene sequence. The CP gene was inserted into the double enzyme digestive pET30a to construct the prokaryotic expression vector,and the recombinant protein was expressed after transformed into Escherichia coli BL-21. [ Result] The size of the obtained CP gene was 1 122 bp,and the similarity with the NCBI target gene exceeded 99% ; the prokaryotic expression vector pET30a-BPMV CP was constructed successfully, and it found that the recombinant protein had highest expression level when it was induced at 37 ℃ for 4 h with 1 mmol/L IPTG. [ Conclusion ] The study laid the foundation for the preparation of BPMV antise-rum and construction of liquid phase chip detection method.关键词
菜豆荚斑驳病毒/外壳蛋白/表达Key words
Bean pod mottle virus/ Coat protein/ Expression分类
农业科技引用本文复制引用
魏晓棠,白桦,尼秀媚,张京宣,宋涛..菜豆荚斑驳病毒外壳蛋白基因的克隆与原核表达[J].安徽农业科学,2013,41(5):1916-1917,2164,3.基金项目
国家质检总局项目(2011IK170). （2011IK170）
