安徽农业科学2013，Vol.41Issue(5)：1937-1938,2.
枯草芽孢杆菌生防菌产孢条件的优化
Optimization on Sporulation Conditions of Biocontrol Bacteria Bacillus subtilis
摘要
Abstract
[Objective] The aim was to explore the optimal sporulation conditions of biocontrol bacteria Bacillus subtilis. [Method] The fermentation medium and conditions of Bacillus subtilis were optimized by using single factor experiment and orthogonal experimental design. [ Result ] The optimal fermentation medium was as followed; 1.500% glucose, 1.000% starch, 1.500% peanut cake,0.005% MnSO4 · H2O,0.050% Mg-SO4,0.150% KH2PO4,0.300% K2HPO4 · 3H2O and 0.050% CaCO3. The optimal fermentation conditions were as followed: culture transferring time 18 -20 h,temperature 37 ℃,initial pH 7.5 .rotational speed 250 r/min,volume of inoculation 10%. [ Conclusion] The research result provides reference for improving the yield of Bacillus subtilis and decreasing production cost.关键词
枯草芽孢杆菌/培养基/发酵条件/优化Key words
Bacillus subtilis/ Medium/ Fermentation conditions/ Optimum分类
农业科技引用本文复制引用
李忠,乐峰松,殷红福,刘华玉..枯草芽孢杆菌生防菌产孢条件的优化[J].安徽农业科学,2013,41(5):1937-1938,2.
