北京大学学报（医学版）2013，Vol.45Issue(2)：221-226,6.DOI:10.3969/j.issn.1671-167X.2013.02.012
顺铂通过诱导膀胱癌细胞自噬促进细胞凋亡
Cisplatin enhances apoptosis in bladder cancer cells via autophagy
摘要
Abstract
Objective:To monitor the cisplatin-induced autophagy and investigate the function of auto-phagy in bladder cancer cells. Methods: The transmission electron microscope was used to detect auto-phagic vacuoles and the fluorescence microscope to detect GFP-LC3. The expressions of proteins, such as LC3, PARP, mTOR, P70S6K were analyzed by immunoblotting. Cell viability was analyzed by MTS assay , in which rapamycin was used to inhibit mTOR phosphorylation and enhance autophagy. LC3 expression was knocked down by RNA interference. Results: In bladder cancer cell T24, autophagic vacuoles were observed by the transmission electron microscope and GFP-LC3 aggregation was viewed by the fluorescence microscope after cisplalin treatment. The LC3- Ⅱ accumulation was enhanced by cispaltin treatment. Particularly at the concentrations of 50, and 100 μmol/L for 48 h , the gray value of LC3-Ⅱ/Ac-tin (% ) increased 30 and 44, respectively. Cisplatin treatment inhibited the phosphorylation of mTOR/ P70S6K, which was most significant at the concentration of 100 μmol/L for 48 h. Cisplatin also induced cell viability loss, which was 12% and 45% at the concentrations of 50, and 100 μmol/L for 24 h. This effect could be enhanced by rapamycin ( F = 74. 890, P < 0. 01 ) . Furthermore, knocking down LC3 by RNA interference reduced PARP cleavage. Conclusion: Cisplatin could induce autophagy in bladder cancer cell T24, which promoted cisplatin-induced apoptosis.关键词
顺铂/膀胱肿瘤/自噬/细胞凋亡Key words
Cisplatin/ Bladder neoplasms/ Autophagy/ Apoptosis分类
医药卫生引用本文复制引用
杨轩,袁栋栋,姜学军,席志军..顺铂通过诱导膀胱癌细胞自噬促进细胞凋亡[J].北京大学学报（医学版）,2013,45(2):221-226,6.基金项目
国家自然科学基金(81272829)资助 （81272829）
