重庆医学2013，Vol.42Issue(12)：1374-1376,3.DOI:10.3969/j.issn.1671-8348.2013.12.021
木犀草素抑制microRNA-21的表达诱导乳腺癌细胞凋亡
Luteolin induced apoptosis in breast cancer cell by decreasing the expression of microRNA-21
摘要
Abstract
Objective To observe the anticancer activity of luteolin on breast cancer cells,and investigate the role of microRNA 21(miR-21) in the effect. Methods Cells viability was assessed by MTT method. Cell apoptosis was analyzed by using Annexin V/ PI dyeing by flow cytometry. The level of Cyt C was tested by Western blot. miR-21 was evaluated by real time quantitative reverse transcription PCR(RT PCR).The plasmids expressing miR-21 was constructed to up regulate the expression of miR-21 in breast cancer cells. Results Luteolin significantly inhibited the proliferation of breast cancer cell MDA MB 453. The cytometry analysis and Western blot detection showed luteolin markedly induced apoptosis in breast cancer cells through increasing the level of Cyt C. Further studies indicated that luteolin decreased the expression of miR-21, and the up regulation of miR-21 could significantly re duce the pro apoptosis effect of luteolin on breast cancer cells. Conclusion Luteolin could significantly inhibit the proliferation and induce apoptosis in breast cancer cells,which could inhibit the expression of miR-21.关键词
木犀草素/乳腺肿瘤/microRNA-21/细胞凋亡Key words
luteolin/breast neoplasms/microRNA-21/ apoptosis引用本文复制引用
王丽娟,王静..木犀草素抑制microRNA-21的表达诱导乳腺癌细胞凋亡[J].重庆医学,2013,42(12):1374-1376,3.
