福建农业学报2013，Vol.28Issue(1)：1-4,4.
两株不同疾病型鸭呼肠孤病毒部分生物学特性的比较
Comparison of Biological Characteristics of Two Different Disease-type Duck Reovirus (MDRV and NDRV)
摘要
Abstract
Muscovy duck reovirus (MDRV) and Novel duck reovirus (NDRV), which belong to Orthoreovirus, are important pathogens of waterfowl. This study was conducted to compare the biological characteristics of MDRV and NDRV. Several methods were undertaken such as ultrathin sections observation, cross virus-neutralization test, the pathogenicity to avian embryos and cell lines, comparison of genome segment profiles and the characteristics analysis of S3 gene. The results of ultrathin sections observation showed that MDRV and NDRV viral particles were spherical and the diameter was both about 70 nm. MDRV scattered in MDEF cytoplasm, whereas NDRV particles arranged in crystal lattice. Relatedness (R) values showed that the antigen of the two viruses were generally different. MDRV's CPE feature was cell rounding, while which of NDRV was cell fusion. NDRV induced the death of 9-day-old chicken embryos, whereas MDRV did not. The migration profiles of NDRV and MDRV genome segments were different. The S3 genes of the two viruses were at different branches of molecular phylogenetic tree. In conclusion, the biological characteristics of MDRV and NDRV were obviously different.关键词
番鸭呼肠孤病毒/新型鸭呼肠孤病毒/生物学特性Key words
Novel duck reovirus/ Muscovy duck reovirus/ biological characteristics分类
农业科技引用本文复制引用
陈仕龙,陈少莺,林锋强,王劭,程晓霞,朱小丽,李兆龙,王锦祥..两株不同疾病型鸭呼肠孤病毒部分生物学特性的比较[J].福建农业学报,2013,28(1):1-4,4.基金项目
国家"863"计划项目(2011AA10A209) （2011AA10A209）
福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1025-5) （2010R1025-5）
国家自然科学基金项目(31172334) （31172334）
