福建农业学报2013，Vol.28Issue(2)：101-106,6.
水稻OsAPX基因的克隆及过表达载体的构建
Cloning and Construction of Over-expression Vector of OsAPX Gene
摘要
Abstract
A full length cDNA of ascorbate peroxidase (APX) gene was cloned from Oryza sativa L. Japonica Nipponbare with RT-PCR. It was 768 bp including the CDS, and was connected to the plant expression binary vector, pBI121, to construct an over-expression vector of APX gene. The pBI121-APX vector was transformed into indica rice, Duoxi No. 1 and Hang No. 1, mediating by Agrobacterium tumefaciens EHA105. With this, transgenic plants could be obtained for further study on the function of APX gene in rice storability.关键词
克隆/APX/过表达载体/遗传转化Key words
clone/ APX/ over-expression vector/ genetic transformation分类
农业科技引用本文复制引用
许惠滨,连玲,魏毅东,朱永生,谢华安,王宗华,张建福..水稻OsAPX基因的克隆及过表达载体的构建[J].福建农业学报,2013,28(2):101-106,6.基金项目
国家转基因农作物新品种培育重大专项(2008ZX08001-006、2011ZX08001-006) （2008ZX08001-006、2011ZX08001-006）
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y04) （STIF-Y04）
