福建农业学报2013，Vol.28Issue(2)：158-165,8.
响应面优化琼脂糖的EDTA-Na2法提取工艺
EDTA-Na2 Extraction of Agarose with Response Surface Optimization
摘要
Abstract
This paper studied the technique of agarose extraction with EDTA - Na2 method. The main factors discussed were:EDTA - Na2 concentration, extraction temperature and extraction time on influencing in sulfate radical content and gel strength. Using the response surface method to optimize the extraction process, the best parameters were EDTA - Na2 concentration 4. 5 g · L-1 , extraction temperature 65℃ and extracting time 3 h. With the optimized parameters, the sulfate radical content and gel strength of agarose extracted were 0. 48% and 1 073. 09 g · cm-2 , respectively. The optimal extraction parameters were verified by repetitive tests with relative error < 1. 0%. It is suggested that EDTA - Na2 method is accurate and repeatable in using for agarose extraction.关键词
响应面法/EDTA-Na2法/琼脂糖Key words
Response surface method/ EDTA - Na2 method/ agarose分类
轻工纺织引用本文复制引用
李龙,苏永昌,吴成业..响应面优化琼脂糖的EDTA-Na2法提取工艺[J].福建农业学报,2013,28(2):158-165,8.基金项目
国家海洋公益性行业科研专项(201205022-4) （201205022-4）
