环境工程学报2013,Vol.7Issue(4):1589-1593,5.
润滑油高效降解菌的筛选及降解性能
Screening of lube oil biodegradation strain and degrading characteristics
摘要
Abstract
With oil contaminated soil as starting bacterial source and lube oil as sole carbon source, 4 lube oil biodegradation strains were screened. 4 strains were identified as Xanthomonas, Azotobacter, Pseudomonas and Flavobacterium separately, according to their morphological, physiological, and biochemical features. G4 in 4 screened strains had the highest biodegradation rate. Degrading characteristics of strain G4 were studied and a-mong the experimental factors, the culture temperature had the greatest effect on biodegradation rate, followed by the concentration of glucose, the concentration of ammonium sulphate, and pH. Strain G4 could degrade lube oil in the temperature range of 20 ~40℃. The degradation rate was over 80% in the pH range of 5.0 ~9.0 at appropriate temperature. When 200 μL lube oil, the sole carbon source, was degraded by 100 mL strain culture medium for 36 h, the biodegradation rate rose to 84. 6% under the optimum conditions as follows; ammonium sulphate concentration of 1. 0 g/L, pH of 9.0 and 30℃.关键词
润滑油/生物降解菌/降解性能Key words
lube oil/ biodegradation strain/ degrading characteristics分类
资源环境引用本文复制引用
郭晓燕,张志红,沈齐英,李翠清..润滑油高效降解菌的筛选及降解性能[J].环境工程学报,2013,7(4):1589-1593,5.基金项目
北京市优秀人才培养资助D类项目(2011D005005000002) (2011D005005000002)