色谱2013，Vol.31Issue(2)：117-121,5.DOI:10.3724/SP.J.1123.2012.10005
固相萃取-高效液相色谱-串联质谱联用测定竹笋中残留的7种杀虫剂农药
Determination of seven pesticide residues in bamboo shoots by high performance liquid chromatography-tandem mass spectrometry coupled with solid-phase extraction
摘要
Abstract
A simple and efficient method based on solid-phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the determination of butylene fipronil, chlorpyrifos, chlorantraniliprole, fipronil, imidacloprid, indoxacarb and phoxim residues in bamboo shoots. The sample was extracted with acetonitrile and purified by a primary secondary amine (PSA) solid-phase extraction cartridge. The detection of targets was performed by HPLC-MS/MS with multiple reaction monitoring (MRM) mode. All the seven pesticides were completely separated in 15 min with an excellent linear relationship. The limits of detection and the limits of quantification for the seven pesticides were 0. 02 -0. 5 μg/kg and 0. 08 -1.5 μg/kg, respectively. The recoveries of the seven pesticides spiked in a bamboo shoot sample at the levels of 4, 8, 32 μg/kg were in the range from 76. 0% to 102. 6% (the RSD ≤ 11. 0%, n = 3). The method was successfully applied to the real bamboo shoot samples. The method has high accuracy and sensitivity, and is simple and quick. It can meet the requirement of the simultaneous determination of the seven pesticides in bamboo shoots.关键词
高效液相色谱-串联质谱/农药残留/竹笋Key words
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS)/ pesticide residues/ bamboo shoots分类
化学化工引用本文复制引用
丁明,钟冬莲,汤富彬,方伟..固相萃取-高效液相色谱-串联质谱联用测定竹笋中残留的7种杀虫剂农药[J].色谱,2013,31(2):117-121,5.基金项目
国家林业局林业公益性行业科研专项经费(201204414). （201204414）
