色谱2013，Vol.31Issue(2)：170-173,4.DOI:10.3724/SP.J.1123.2012.09035
分散固相萃取-高效液相色谱法快速测定果蔬中的6种保鲜剂残留量
Rapid determination of six preservative residues in fruits and vegetables by high performance liquid chromatography using dispersive solid phase extraction
摘要
Abstract
A dispersive solid phase extraction-high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of 2,4-D, thiabendazole, 2-naphthol, o-phenyl phenol, diphenyl ether and biphenyl in the fruits and vegetables. The samples were vortexed for extraction with acetonitrile containing sodium chloride and anhydrous magnesium sulfate. The acetonitrile extraction was purified by acidic alumina dispersed solid phase extraction. The analysis was performed on an Agilent TC Cl8 column (250 mm×4. 6 mm, 5 μm) and an ultraviolet detector at 235 nm with a mobile phase of methanol-0. 02 mol/L (pH 6) potassium dihydrogen phosphate solution, at a flow rate of 1. 0 mL/min with gradient elution. Good linearities were observed in the range of 0. 5 - 20 mg/L with correlation coefficients greater than 0. 99. The average recoveries of the six preservatives in fruits and vegetables were in the range of 84. 2% - 99. 1% at three spiked levels of 1, 2 and 10 mg/kg (n = 6) and the relative standard deviations (RSDs) were in the range of 1. 67% - 10. 3%. The limits of quantification were 1 mg/kg. This method is simple, accurate and suitable for the determination of the six preservatives in fruits and vegetables.关键词
高效液相色谱/分散固相萃取/保鲜剂/果蔬Key words
high performance liquid chromatography ( HPLC)/ dispersive solid phase extraction/ preservatives/ fruits and vegetables分类
化学化工引用本文复制引用
林海丹,邹志飞,秦燕,谢守新,陈毓芳,徐娟,王岚,陈楚莹..分散固相萃取-高效液相色谱法快速测定果蔬中的6种保鲜剂残留量[J].色谱,2013,31(2):170-173,4.基金项目
国家质检总局公益性行业科研专项(201110019) （201110019）
中国检验检疫科学研究院项目(2009JK011) （2009JK011）
"十二五"农村领域国家科技计划课题(2011BAD23B05-4) （2011BAD23B05-4）
广东省科技基础条件建设项目(粤科财字[2008]658,[2010]185). （粤科财字[2008]658,[2010]185）
