山西大学学报（自然科学版）2013，Vol.36Issue(1)：113-117,5.
硫化氢对拟南芥在干旱胁迫条件下的生理影响
Physiological Effects of Hydrogen Sulfide under Drought Stress in Arabidopsis thaliana
摘要
Abstract
Arabidopsis thaliana wild-type (WT) and knockdown mutant of LCD,a L-cysteine desulfhydrase encoding gene to catalyze the reaction to produce H2S in vivo,were used as experimental materials. The result indicated the effect of endogenous H2S on seed germination and seedling growth under drought stress. Moreover, the results showed that the seed germination was inhibited significantly in led compared to WT under drought stress, with germination rate decreasing by about 40%; the MDA content of the seedlings was more 1. 56 times in lcd than that in WT with extremely significant difference; at the same time the H2O2 content also increased significantly. Under the normal conditions,all antioxidant enzyme activities in lcd and WT showed similar values. The POD, CAT, SOD and APX activity enhanced about 2. 11,1. 48, 2. 22 and 1. 37 folds in lcd after drought stress,respectively,which showed significant difference compared with that in WT. All together,these results suggest that endogenous H2S as gasotransmitter can affect the seed germination and seedling development through regulation of antioxidant system in A. thaliana.关键词
拟南芥/硫化氢/干旱胁迫/种子萌发/抗氧化酶Key words
Arabidopsis thaliana /hydrogen sulfide/drought stress/seed germination/antioxidant enzymes分类
农业科技引用本文复制引用
金竹萍,方慧慧,张丽萍,罗雅楠,乔增杰,龚泽华,裴雁曦..硫化氢对拟南芥在干旱胁迫条件下的生理影响[J].山西大学学报（自然科学版）,2013,36(1):113-117,5.基金项目
山西省回国留学人员项目(2011-007) （2011-007）
山西省教育厅山西省优秀青年学术带头人支持计划(200906) （200906）
教育部博士点(博导类)项目(20091401110004) （博导类）
