实用医学杂志2013，Vol.29Issue(6)：992-994,3.DOI:10.3969/j.issn.1006-5725.2013.06.052
阿尔茨海默病患者脑脊液miRNA检测及初步分析
MicroRNA profiling in cerebrospinal fluid in patients with Alzheimer's disease and preliminary analysis
摘要
Abstract
Objective To determine the microRNA(miRNA) expression profile of in CSF in AD patients. Methods miRNA microarrays were performed to detect the expression profile of miRNAs in 10 cases of CSF in AD patients and the healthy control persons. Preliminary bioinformatic analysis was conducted. The microarray results were confirmed by real-time PCR assay. Results miRNA microarray results revealed that 128 miRNAs were dysregulated in AD patients and the corresponding healthy controls. Sixty-three miRNAs were significantly up-regulated, and 65 miRNAs were significantly down-regulated. Real-time PCR results demonstrated that up-regulation of miR-125b and miR-132 was consistent with that based on microarray array. Conclusion Specific miRNA expression profile was determined in CSF in AD patients, and the differentially expressed miRNAs may be used as potential biomarkers of AD.关键词
微小RNA/阿尔茨海默病/脑脊液/基因芯片/Real-Time PCRKey words
microRNA/ AD/ Cerebrospinal fluid/ Microarray/ Real-time PCR引用本文复制引用
胡国艳,李萍,何柯新,徐鹏,罗新妮,钟笑梅,侯乐,宁玉萍,刘学军..阿尔茨海默病患者脑脊液miRNA检测及初步分析[J].实用医学杂志,2013,29(6):992-994,3.基金项目
国家自然科学基金项目(编号:30970902) （编号:30970902）
广东省科技计划项目(编号:2010B031600018) （编号:2010B031600018）
广州市科技计划项目(编号:2010Y1-C631) （编号:2010Y1-C631）
