应用生态学报2013，Vol.24Issue(4)：1023-1030,8.
外源NO对铜胁迫下番茄幼苗根系抗坏血酸-谷胱甘肽循环的影响
Effects of exogenous nitric oxide on ascorbate-glutathione cycle in tomato seedlings roots under copper stress
摘要
Abstract
By using solution culture method, this paper studied the effects of exogenous nitric oxide ( NO ) on the antioxidants and antioxidases in the ascorbate-glutathione ( AsA-GSH) cycle in tomato ( Lycopersicon esculentum Mill. ) seedling roots under copper stress. Exogenous NO could affect the metabolic cycle of AsA-GSH in tomato roots under copper stress. Applying appropriate amount of exogenous NO increased the AsA and GSH contents, AsA/DHA and GSH/GSSG ratios, and decreased the DHA and GSSG contents in tomato roots under copper stress. With the addition of 100 μmol·L-1 of BSO, exogenous NO increased the AsA content, AsA/DHA ratio, and the AAO, MDHAR, and DHAR activities, and decreased the DHA, GSH, and GSSG contents and the APX and GR activities. When 250 μmol·L-1 of BSO was added, exogenous NO increased the contents of AsA, GSH, and GSSG, AsA/DHA ratio, and the activities of APX and GR, and decreased the DHA content and the AAO, DHAR and MDHAR activities. It was suggested that exogenous NO could affect the metabolic cycle of AsA-GSH in tomato roots under copper stress, and mitigate the damage of copper stress to tomato roots via regulating the AsA/DHA and GSH/GSSG ratios to alleviate oxidative stress.关键词
一氧化氮/番茄/铜胁迫/抗坏血酸-谷胱甘肽循环Key words
nitric oxide/ tomato/ copper stress/ ascorbate-glutathione cycle分类
农业科技引用本文复制引用
李晓云,王秀峰,吕乐福,尹博,张敏,崔秀敏..外源NO对铜胁迫下番茄幼苗根系抗坏血酸-谷胱甘肽循环的影响[J].应用生态学报,2013,24(4):1023-1030,8.基金项目
国家自然科学基金项目(31201619)、泰安市科技发展计划项目(32606)和现代农业技术体系建设专项基金(CAR-25-D)资助. （31201619）
