湖北医药学院学报2013，Vol.32Issue(1)：39-43,封2,6.DOI:10.7543/j.issn.1006-9674.2013.01.007
绞股蓝皂苷降低2型糖尿病并非酒精性脂肪性肝病大鼠血糖、血脂的机理研究
The Mechanism of Blood Sugar,Triglycerides and Total Cholesterol Reduced by Gypenoside in Rats with Type 2 Diabetes Mellitus and Nonalcohol Fatty Liver Disease
摘要
Abstract
Objective To investigate the mechanism of blood sugar,triglycerides and total cholesterol reduced by gypenoside in rats with type 2 diabetes mellitus and nonalcohol fatty liver disease( NAFLD). Methods Sixty-five SPF male SD rats body weight 220 to 250 g,were randomly divided into blank control group (group N,n =7) , NAFLD model group (group NM,n = 7) ,NAFLD combined T2DM model group(n =51). The NAFLD model,NAFLD combined T2DM model of rats were prepared. The survival NAFLD combined T2DM model rats were further divided into group JH,group JL and group M(n =9). The rats of group JH were given gypenoside with a dose of 1g/(kg·d),the rats of group JL were given gypenoside with a dose of 0.5 g/( kg·d) ,and the rats in group M were given distilled water. The changes of peroxisome proliferator-activated receptor/(PPARγ) ,blood sugar(BS) ,triglycerides(TG) ,total cholesterol(TC) in liver tissue were determined by quantitative PCR. The pathological changes of liver tissue was observed with HE staining. Results The intervention of gypenoside significantly decreased the levels of BS, TG, TC in NAFLD combined T2DM model rats, up-regulated the expression of PPARγ, decreased the infiltration of liver fats in a dose-dependent manner. Conclusion Gypenoside could decrease the BS, TG,TC levels in NAFLD combined T2DM model rats through up-regulating the expression of PPAR7 to interfere with their metabolism.关键词
2型糖尿病/非酒精性脂肪性肝病/血糖/甘油三酯/胆固醇/过氧化物酶体增殖物激活受体γ/绞股蓝皂苷Key words
Type 2 diabetes mellitus/Nonalcoholic fatty liver disease/Blood sugar/Triglycerides/Total cholesterol/Peroxi-some proliferator-activated receptory/ Gypenoside引用本文复制引用
贺琴,雷飞飞,李儒贵,谭华炳..绞股蓝皂苷降低2型糖尿病并非酒精性脂肪性肝病大鼠血糖、血脂的机理研究[J].湖北医药学院学报,2013,32(1):39-43,封2,6.基金项目
2009年湖北省卫生厅科研项目(JX4B58),2008年十堰市科学技术研究与开发项目(2008024D) （JX4B58）
