中国病理生理杂志2013，Vol.29Issue(4)：584-589,6.DOI:10.3969/j.issn.1000-4718.2013.04.002
干扰素诱导蛋白16对人脑血管外膜成纤维细胞增殖与迁移的影响及其机制
Effects of interferon-inducible protein 16 on proliferation and migration of human brain vascular adventitial fibroblasts
摘要
Abstract
AIM: To investigate the effects of interferon-inducible protein 16 (IFI16) on the proliferation and migration of human brain vascular adventitial fibroblasts (HBVAFs). METHODS: The siRNA of IFI16 gene was trans-fected into HBVAFs. Forty-eight hours after transfection, the cells were exposed to 2 x 106 U/L interferon alpha (IFN-α) for 24 h. Cell cycle was analyzed by flow cytometry. Cell migration was determined by scratch assay and transwell method. The mRNA and protein levels of IFI16, p53 and p21 were measured by real-time PCR and Western blotting, respectively. RESULTS; After transfection with IFI16 siRNA, the expression of IFI16, p53 and p21 at mRNA and protein levels was decreased in HBVAFs, and the cell cycle at G1/S transition was promoted. Meanwhile, stimulated with IFN-α up-regulated the expression of IFI16, p53 and p21 at mRNA and protein levels, and inhibited the cell cycle transition at G1/S and cell migration in HBVAFs. Such effect was restrained by transfection with IFI16 siRNA into HBVAFs. CONCLUSION: The expression of IFI16 inhibits the proliferation and migration of HBVAFs, which may be related to the activation of p53 and p21 expression.关键词
干扰素诱导蛋白/血管外膜成纤维细胞/细胞增殖/细胞迁移Key words
Interferon-inducible protein/Vascular adventitial fibroblasts/Cell proliferation/Cell migration分类
医药卫生引用本文复制引用
黄晶,宋方,龙向淑,吴强..干扰素诱导蛋白16对人脑血管外膜成纤维细胞增殖与迁移的影响及其机制[J].中国病理生理杂志,2013,29(4):584-589,6.基金项目
国家自然科学基金资助项目(No.81260030) （No.81260030）
贵州省高层次人才科研条件特助经费项目合同(No.TZJF-2010年-098号) （No.TZJF-2010年-098号）
贵州省科技攻关项目(黔科合LS字[2012]021号) （黔科合LS字[2012]021号）
