中国病理生理杂志2013，Vol.29Issue(4)：707-712,6.DOI:10.3969/j.issn.1000-4718.2013.04.023
骨髓间充质干细胞在小鼠早衰性骨质疏松中的细胞生物学功能研究
Age-related changes of bone marrow mesenchymal stem cells in senile osteoporosis
摘要
Abstract
AIM: To investigate the different functions of bone marrow mesenchymal stem cells ( BMMSCs) in age-related osteoporosis. METHODS: The senescence-accelERαted mice (SAMP6) were used in the experiment. The BMMSCs were isolated from femora and tibiae by flushing. Flow cytometric analysis was performed with MSCs-related monoclonal antibodies. The expression of differentiation genes was tested by real-time RT-PCR. RESULTS: In the progress of age-related osteoporosis, BMMSCs exhibited a decrease in osteogenesis and an increase in adipogenesis. Transforming growth factor β(TGF-β) signaling was significantly changed along with aging in SAMP6 mice. CONCLUSION: The functional changes of BMMSCs may play an important role in senile osteoporosis. The altERαtion of TGF-β-related gene expression may be the molecular mechanism of dysfunction in BMMSCs.关键词
骨髓间充质干细胞/骨质疏松/衰老/细胞分化Key words
Bone marrow mesenchymal stem cells/Osteoporosis/Aging/Cell differentiation分类
医药卫生引用本文复制引用
杲丽,胡成虎,金岩..骨髓间充质干细胞在小鼠早衰性骨质疏松中的细胞生物学功能研究[J].中国病理生理杂志,2013,29(4):707-712,6.基金项目
国家重点基础研究发展计划项目(No.2011CB964700) （No.2011CB964700）
