中国病理生理杂志2013，Vol.29Issue(4)：713-717,5.DOI:10.3969/j.issn.1000-4718.2013.04.024
p38 MAPK抑制剂SB203580对雨蛙肽诱导的小鼠胰腺组织损伤的影响
Effect of p38 MAPK inhibitor SB203580 on caerulein-induced pancreatic tissue injury in mice
摘要
Abstract
AIM: To investigate the effect of p38 MAPK inhibitor SB203580 (SB) on caerulein (CAE)-induced injury in isolated mouse pancreatic tissues. METHODS: The pancreatic tissues of the mice were isolated, cultured for 4 h, and stimulated with CAE (10-5mol/L) alone or combined with SB (10-5mol/L). Normal saline (NS) was used as control reagent. At the time points of 1 h and 4 h after stimulation, the pancreatic tissues and the culture supernatants were harvested. The following parameters for evaluating the degree of injury were observed: the viability of the pancreatic tissues, the activity of amylase and lipase, and the content of interleukin-6 (IL-6) and cytokine-induced neutrophil chemo-tactic factor 1 (CINC-1) in the cultured supernatant. The levels of heat-shock protein (HSP) 60 and 70, and p38 and p-p38 proteins in the pancreatic tissues were measured by ELISA or Western blotting. RESULTS: After stimulation with CAE, the viability of the pancreatic tissues decreased at 1 h and was even lower at 4 h. The levels of amylase, lipase, IL-6, CINC-1, HSP60 and HSP70 as well as the expression of p38 and p-p38 were significantly increased at the time point of 1 h as compared with NS group. These changes were partly or totally prevented by SB treatment. CONCLUSION: CAE effectively damages the isolated pancreatic tissues. SB203580 reduces the injury, which may be related to the inhibition of the inflammatory responses.关键词
胰腺/p38 MAPK信号通路/雨蛙肽Key words
Pancreas/p38 MAPK signaling pathway/Caerulein分类
医药卫生引用本文复制引用
徐菁,曹明华,冯雅静,李琨,张一真,李永渝..p38 MAPK抑制剂SB203580对雨蛙肽诱导的小鼠胰腺组织损伤的影响[J].中国病理生理杂志,2013,29(4):713-717,5.基金项目
国家自然科学基金资助项目(No.81270477) （No.81270477）
