中国病理生理杂志2013，Vol.29Issue(4)：724-729,6.DOI:10.3969/j.issn.1000-4718.2013.04.026
壳聚糖/液晶复合水凝胶的制备及细胞相容性研究
Preparation and cytocompatibility of chitosan/liquid crystal composite hydrogel
摘要
Abstract
ATM: To construct chitosan/liquid crystal (CS/LC) composite hydrogel based on the biomimetic strategy. METHODS: The CS/LC composite hydrogel with an LC-based structure that was similar to the biomembrane was prepared by the composition of CS matrix with hydroxypropyl cellulose derivative LC possessing the feature of fluidity and ordering. The effect of microstructure of the composite hydrogel on the cell behavior was studied by investigating the morphology, phase separation structure and cell compatibility. The composite hydrogel was characterized by scanning electron microscope and X-ray diffraction, and was evaluated by cell viability test. RESULTS: The CS/LC composite hydrogel presented amorphous state, and the LC domain formed island-like distribution among the CS substrate, which resulted in uniformly phase separation between LC and CS. Cell compatibility test showed that the composite hydrogel contai-ning continuously dispersed LC domains favored the cell initial adhesion and growth. CONCLUSION: Biomimetic CS/LC composite hydrogel with tissue-like viscoelasticity is excellent in cell compatibility.关键词
壳聚糖/液晶/水凝胶/细胞培养Key words
Chitosan/Liquid crystals/Hydrogel/Cell culture分类
生物科学引用本文复制引用
司徒方民,韩婉清,屠美,查振刚,曾戎,叶菁芸,吴昊,张嘉晴,周长忍..壳聚糖/液晶复合水凝胶的制备及细胞相容性研究[J].中国病理生理杂志,2013,29(4):724-729,6.基金项目
国家自然科学基金资助项目(No.31170911) （No.31170911）
暨南大学附属第一医院开放研究基金资助项目 （）
