中国病理生理杂志2013，Vol.29Issue(4)：743-747,5.DOI:10.3969/j.issn.1000-4718.2013.04.030
二苯乙烯苷对同型半胱氨酸诱导血管内皮细胞凋亡及bcl-2、bax、caspase-3表达的影响
Effects of tetrahydroxystilbene-2-O-β-D-glucoside on apoptosis and expression of bcl-2/bax/caspase-3 in HUVECs treated with homocysteine
摘要
Abstract
AIM: To explore the effects of tetrahydroxystilbene-2-O-β-D-glucoside (TSG) from Polygonum multiflorum on the apoptosis and the mRNA expression of bcl-2, bax and caspase-3 in human umbilical vein endothelial cells (HUVECs) treated with homocysteine (Hey). METHODS: Cultured HUVECs were treated with Hey (3 mmol/L) to establish a Hcy-damaged model. HUVECs in TSG treated groups were pre-incubated with TSG at concentrations of 1 μmol/L and 10 μmol/L for 2 h before treated with Hey. Cell nuclear damage was detected by Hoechst 33342 staining. Cell apoptosis was determined by flow cytometry. The mRNA expression of bcl-2, bax and caspase-3 was measured by real-time fluorescence quantitative RT-PCR. RESULTS: After treatment with Hey at concentration of 3 mmol/L, the nuclear damage and apoptotic rate of HUVECs were higher than that in normal group. The expression of bcl-2 was lower, and the expression of Bax and caspase-3 was higher than that in normal group. On the other hand, pre-incubation with TSG at concentrations of 1 μmol/L and 10 μmol/L decreased the nuclear damage and cell apoptosis, increased the expression of bcl-2, and decreased the expression of bax and caspase-3 as compared with the cells only treated with Hey. CONCLUSION: TSG reduces the apoptosis of HUVECs induced by Hey, and the mechanism might be associated with regulating the expression of bcl-2, bax and caspase-3.关键词
二苯乙烯苷/同型半胱氨酸/细胞凋亡/Bcl-2/Bax/半胱氨酸天冬氨酸蛋白酶3Key words
Tetrahydroxystilbene-2-O-3-D-glucoside/Homocysteine/Apoptosis/Bcl-2/Bax/Caspase-3分类
医药卫生引用本文复制引用
李军,王国荣,张秀芹,王燕,牟艳玲,姚庆强..二苯乙烯苷对同型半胱氨酸诱导血管内皮细胞凋亡及bcl-2、bax、caspase-3表达的影响[J].中国病理生理杂志,2013,29(4):743-747,5.基金项目
山东省自然科学基金资助项目(No.ZR2008C317) （No.ZR2008C317）
